JCRB No. JCRB0149 Cell Name Bhas 42
Profile BALB 3T3 A31-1-1 cell line transfected with H-ras gene. The cell line keeps characteristics of the contact inhibition and the transfomation is induced by phorbol ester alone. No initialization required. (Strain problem was found. Refer Important Notice at cell detail page.) Other Name
Animal mouse Strain Swiss
Genus Mus Species musclus
Sex Age embryo
Identity Tissue for Primary Cancer embryo
Case history normal Metastasis
Tissue Metastasized Genetics H-ras gene transfected BALB/c 3T3 A31-1-1 cell line. The origin of BALB/c 3T3 A31-1 was found not to be derived from BALB 3T3 A31.
Life Span infinite Crisis PDL
Morphology fibroblast-like Character Growth on the dish is inhibited by cell to cell contact. Phorbol ester alone is enough to induce cellular transformation.
Classify transfected Established by Sasaki,K.
Registered by Sasaki,K. Regulation for Distribution free
Comment Appearing of naturally occured transformation depends on serums and passage methods. Year 2000
Medium Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio is practically 1/100. Harvest cells before confluent at subculture.
Cell Number on Passage 2 x 10^4 cells/100mm dish. Race
CO2 Conc. 5 % Tissue Sampling whole body of embryo
Tissue Type