INNOTEST sTREM2 is a solid-phase enzyme immunoassay for the quantitative determination of soluble Triggering Receptor Expressed on Myeloid cells 2 (sTREM2) in human cerebrospinal fluid (CSF).
For research use only
Details
This assay should be used in combination with the sTREM2 CAL-RVC pack, article no. 81057 (RUO), of the corresponding lot.
Test principle
The INNOTEST sTREM2 is a solid-phase enzyme immunoassay in which the sTREM2 protein is captured by a first monoclonal antibody, ADx601 (B02; rabbit; IgG). CSF samples are added and incubated with a biotinylated antibody, ADx602 (C10; rabbit; IgG). Both ADx601 and ADx602 are directed against the ectodomain of human TREM2 and the epitope of ADx601 is further specified in Figure 1. This antigen-antibody complex is then detected by a peroxidase-labeled streptavidin. After addition of substrate working solution, samples will develop a color. The color intensity is a measure for the amount of human sTREM2 protein in the sample.