The Cylindrospermopsin ELISA is an immunoassay for the quantitative and sensitive detection of Cylindrospermopsin in water samples. No additional sample preparation is required prior to analysis. If necessary, positive samples can be confirmed by HPLC or other conventional methods.
The test is a direct competitive ELISA for the detection of Cylindrospermopsin. It is based on the recognition of Cylindrospermopsin by specific antibodies. Cylindrospermopsin, when present in a sample, and a Cylindrospermopsin-HRP analogue compete for the binding sites of rabbit anti-Cylindrospermopsin antibodies in solution. The anti-Cylindrospermopsin antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Cylindrospermopsin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
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PN 522011
Format: 96-Well Plate ELISA
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