CHO HCP ELISA
The Chinese hamster ovary (CHO) cell line is the most prevalent biopharmaceutical expression system and has been proven safe for the commercial production of protein therapeutics. To ensure the safety of recombinantly expressed biopharmaceuticals, health authorities are demanding the close monitoring of HCP impurities by applying HCP detection methods with high sensitivity and specificity. The BioGenes’ CHO|360-HCP ELISA is an enhanced generic HCP assay composed of four different generic kits, and was designed to cover a broader spectrum of CHO-HCPs compared to single generic kits.
Four CHO Kit Types
The CHO|360-HCP ELISA kits (Type A to D) are based on a unique assay design, employing differently prepared HCP antigens and antibodies from two animal species:
- The antigen is prepared from total and fractionated HCP obtained from mock fermentation of CHO-K1 and CHO-S
- The specific antibodies are generated by parallel immunization of goats and rabbits
Type
|
Species
|
HCP
|
A
|
Goat
|
Total HCP
|
B
|
Goat
|
Fractionated HCP
|
C
|
Rabbit
|
Total HCP
|
D
|
Rabbit
|
Fractionated HCP
|