RAW 264.7 cells

Catalog #:C0003008

Disease:

Abelson murine leukemia virus-induced tumor

Origin:

Established from an ascites of a tumor induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumor targets. Growth inhibited by LPS.

Species:

Mus musculus

Tissue:

Blood

Morphology:

Macrophage/Monocyte

Properties:

Partial Adherent

Patient:

Mouse

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

II

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

References for RAW 264.7 cells:

1. Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977.