JCRB No. JCRB0403 Cell Name HuH-7
Profile Well differentiated human hepato cellular carcinoma cell line. Other Name HuH-7, JTC-39
Animal human Strain
Genus Homo Species sapiens
Sex M Age 57
Identity available Tissue for Primary Cancer liver, gallbladder
Case history Well differentiated hepato cellular carcinoma. Metastasis
Tissue Metastasized Genetics differenciated hepatoma
Life Span infinite Crisis PDL
Morphology epithelial-like Character hepatoma (differenciated), age 57, (M)
Classify tumor Established by Nakabayshi,H. & Sato,J.
Registered by Sato,J. Regulation for Distribution
Comment This cell line can be grown in serum-free medium. Many serum proteins with alpha fetoprotein are released into the medium. Year 1985
Medium Dulbecco's minimal essential medium with 10% fetal bovine serum. Refer Ref {1201} for other medium with low serum concentration. Methods for Passages Cells are treated with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage 2.5x10^4 cells/cm^2 Race Japanese
CO2 Conc. 5 % Tissue Sampling liver